We narrowed to 14,573 results for: cas9

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dCas9-SunTag(10X)-2A-BFP

Plasmid

#227389

Purpose

Adhesion module, dCas9-SunTag

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

dCas9-SunTag

Use

Lentiviral

Mutation

Enzymatically dead Cas9

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUbi_dCas9-BFP-KRAB-gRNA

Plasmid

#221503

Purpose

CRISPRi machinery fish codon optimized expressing plasmid

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Mutation

D10A and H839A point mutations

Promoter

Zebrafish Ubiquitin promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaSpCas9-NLS-6xHis

Plasmid

#207386

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-SunTag (dCas9-SunTag)

Plasmid

#218783

Purpose

Expresses dCAS9-SunTag fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Publication

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-SunTag (dCas9-SunTag)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

Plasmid

#211767

Purpose

dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

Depositor

Ryan Lister

Publication

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pGK and pEF1a

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

Plasmid

#159664

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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SauCas9 PE2*

Plasmid

#169851

Purpose

Mammalian Expression, sauCas9 based prime editor

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SaCas9-N580A-M-MLV

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3 CAS9 IRES-Dsred

Plasmid

#129444

Purpose

expression of FnCas9 and DsRed

Depositor

Oskar Laur

Insert

FnCas9 IRES-Dsred

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -296

Plasmid

#50926

Purpose

U6 driven sgRNA targeting Sox17 -296 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17-296 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Publication

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR1_ccB

Plasmid

#158116

Purpose

deletion hADAR1

Depositor

Emanuela Felley-Bosco

Insert

Adar1 (ADAR Human)

Expression

Mammalian

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmiHiFiCas9

Plasmid

#164832

Purpose

miHiFiCas9 expressing plasmid

Depositor

Eugene Chen

Publication

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miHiFiCas9 (contains Brex27 at sequence 5031-5138).

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

change R 691 to A

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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