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We narrowed to 18,120 results for: multi

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Showing: 4301 - 4320 of 18120 results

  4. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  7. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

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  8. pAC-EHER

    Plasmid
    #53262
    Purpose
    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53262-plasmid-map-sequence-id-82988

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  10. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  12. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Expression
    Bacterial
    Promoter
    AmpR and lpp
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  17. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  18. pQhsCDtRNA-

    Plasmid
    #86015
    Purpose
    Multi-gene expression system with human box C/D proteins with U14
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Available Since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86015-plasmid-map-sequence-id-169486

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  20. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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Showing: 4301 - 4320 of 18120 results