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We narrowed to 18,241 results for: multi

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Showing: 4301 - 4320 of 18241 results

  4. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122183-plasmid-map-sequence-id-241282

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  5. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  6. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Publication
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70763-plasmid-map-sequence-id-240922

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  12. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  14. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Publication
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  18. pQhsCDtRNA-

    Plasmid
    #86015
    Purpose
    Multi-gene expression system with human box C/D proteins with U14
    Depositor
    Hong Li
    Publication
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86015-plasmid-map-sequence-id-169486

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  19. pcDNA3-Lyn-dInPAkt

    Plasmid
    #181834
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-dInPAkt
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181834-plasmid-map-sequence-id-364316

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Showing: 4301 - 4320 of 18241 results