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BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP682

Plasmid

#45454

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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HA-hNPHP1

Plasmid

#122872

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Hoque et al Reproduction. 2021 Jul 8;162(2):129-139.

Insert

human NPHP1 (NPHP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti PKAKTRn-Clover

Plasmid

#177200

Purpose

Lentiviral vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHG7

Plasmid

#188255

Purpose

Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cut-P(11)4

Plasmid

#185785

Purpose

Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Cut-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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438-GST

Plasmid

#97092

Purpose

GST-tev-yORF

Depositor

Chris Jeans

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

GST tag and TEV cleavage site

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-ACME

Plasmid

#186717

Purpose

ACME dual-deaminase CRISPR base editor

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

ACME

Use

CRISPR

Tags

Nucleoplasmin NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHG5

Plasmid

#188253

Purpose

Integrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-TRE-mTurquoise2-WPRE

Plasmid

#104103

Purpose

Expresses mTurquoise2 under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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