We narrowed to 15,976 results for: LEA

1. pGBW-m4046174

   Plasmid

   #145427

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046536

   Plasmid

   #145429

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046281

   Plasmid

   #145431

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046354

   Plasmid

   #145418

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046432

   Plasmid

   #145420

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046272

   Plasmid

   #145421

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046527

   Plasmid

   #145412

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046480

   Plasmid

   #145416

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046504

   Plasmid

   #145404

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046244

    Plasmid

    #145405

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046197

    Plasmid

    #145406

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046183

    Plasmid

    #145407

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046321

    Plasmid

    #145409

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046505

    Plasmid

    #145410

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046186

    Plasmid

    #145402

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046288

    Plasmid

    #145386

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046551

    Plasmid

    #145434

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046311

    Plasmid

    #145425

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046214

    Plasmid

    #145399

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046324

    Plasmid

    #145549

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGBW-m4046333

    Plasmid

    #145544

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pGBW-m4046262

    Plasmid

    #145524

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGBW-m4046416

    Plasmid

    #145388

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046570

    Plasmid

    #145558

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046588

    Plasmid

    #145465

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pHBSTC159

    Plasmid

    #124353

    Purpose

    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    NpuDnaBΔ283 (C1G) mini-intein
    

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Deletion of 283-residue endonuclease domain, C1G

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FAM83B (FAM83BA-c004)

    Plasmid

    #98236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FAM83BA (FAM83B Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW210

    Plasmid

    #113189

    Purpose

    E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    H208A

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DCP2 (DCP2B-c016)

    Plasmid

    #98239

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DCP2B (DCP2 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 5C5C

    Plasmid

    #69964

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM7:DCC014-D04:C211307 (GRM7 Human)
    

    Use

    Baculovirus expression

    Tags

    His and TEV cleavage site

    Promoter

    polyhedrin

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

31. 4Z02

    Plasmid

    #69956

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BRD1:JMC023-A05:C211705 (BRD1 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. pJA46

    Plasmid

    #59928

    Purpose

    gRNA for cleavage at rde-1(D801) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rde-1(D801) gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJA45

    Plasmid

    #59927

    Purpose

    gRNA for cleavage at rde-1(D718) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rde-1(D718) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDXNSMG3

    Plasmid

    #49029

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), EGFP, MYC, a…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDXC3MS

    Plasmid

    #49030

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), MYC, and str…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ANKRA

    Plasmid

    #32877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ANKRA (ANKRA2 Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

37. SPL11p::pCGTAG (SPL11p::NLS-GFP)

    Plasmid

    #27411

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL11 promoter (AT1G27360 Mustard Weed)
    

    Mutation

    SPL11 promoter transcriptionally fused to a nucle…

    Available Since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLIC.B4.BSU37660

    Plasmid

    #11535

    Depositor

    Sung-Hou Kim

    Article

    Xu et al J Struct Funct Genomics. 2005 Dec;6(4):2

    Insert

    phosphotransacetylase
    

    Tags

    HisTag-MBP, TEV cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

39. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. tFucci(CA)5pPBbsr2

    Plasmid

    #197351

    Purpose

    Encoding tFucci(CA)5.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    tFucci(CA)5
    

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-Lyn-AKAR4

    Plasmid

    #61620

    Purpose

    Lipid raft-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    Lyn-AKAR4
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pVITRO1-mCherry-LaminB1

    Plasmid

    #186348

    Purpose

    Nuclear membrane-targeted mCherry expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCherry-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SpyCatcher003

    Plasmid

    #133447

    Purpose

    Expresses SpyCatcher003 in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.4_StrepII_TEV_PP2Ac

    Plasmid

    #217477

    Purpose

    Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    PP2Ac
    

    Tags

    StrepII, TEV

    Expression

    Mammalian

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3-AKAR4-Kras

    Plasmid

    #61621

    Purpose

    Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4-Kras
    

    Tags

    C-terminal targeting sequence from Kras, Cerulean…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. mCherry-LaminA-C-18

    Plasmid

    #55068

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Lamin A (LMNA Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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