We narrowed to 11,549 results for: phen
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Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SR
Plasmid#52162PurposeEncodes module 5 with amino acids 12S-16R for recognition of nt 4CDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 5Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SE
Plasmid#52164PurposeEncodes module 6 with amino acids 12S-16E for recognition of nt 3GDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 6Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase S1226A/ C2029A
Plasmid#59823PurposeAllows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationS1226A/ C2029APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc p31Comet
Plasmid#59833PurposeAllows the integration of myc p31Comet in the genome and Tet-inducible expression.DepositorInsertp31 (MAD2L1BP Human)
TagsMycExpressionMammalianPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL WT 3xFlag-V5
Plasmid#174129PurposeExpresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimizedAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-GW
Plasmid#113456PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsProteinA-mCitrineExpressionMammalianPromoterhUbCAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SE
Plasmid#52152PurposeEncodes module 4 with amino acids 12S-16E for recognition of nt 5GDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 4Available SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8SR
Plasmid#52174PurposeEncodes module 8 with amino acids 12S-16R for recognition of nt 1CDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 8Available SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7CQ
Plasmid#52167PurposeEncodes module 7 with amino acids 12C-16Q for recognition of nt 2ADepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
ExpressionBacterialMutationChanged 12S to 12C and 16E to 16Q in Module 7Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7NQ
Plasmid#52169PurposeEncodes module 7 with amino acids 12N-16Q for recognition of nt 2UDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
ExpressionBacterialMutationChanged 12S to 12N and 16E to 16Q in module 7Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7SYR
Plasmid#52170PurposeEncodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2CDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
ExpressionBacterialMutationChanged 13N to 13Y and 16E to 16R in module 7Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SR
Plasmid#52154PurposeEncodes module 4 with amino acids 12S-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 4Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CYQ
Plasmid#52155PurposeEncodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12C and 13H to 13Y in Module 4Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SE
Plasmid#52160PurposeEncodes module 5 with amino acids 12S-16E for recognition of nt 4GDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 5Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SR
Plasmid#52166PurposeEncodes module 6 with amino acids 12S-16R for recognition of nt 3CDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 6Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD53
Plasmid#172457PurposeExpression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tagDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
FB001
Plasmid#119057PurposePromoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter trpC
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB002
Plasmid#119676PurposeTerminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator tub
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB008
Plasmid#119706PurposeTerminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator trpC
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRD404
Plasmid#163109PurposeExpression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmNeonGreen_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GTSE1(638-720)-GFP
Plasmid#100735PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertGTSE1 (GTSE1 Human)
TagsFKBP and GFPExpressionMammalianMutationC-terminus onlyPromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMini-CAAGs::RFP-DR48
Plasmid#132640PurposeFluorescent reporter to quantify elicitation of the strand annealing mediated repair pathwayDepositorInsertmRFP
ExpressionMammalianMutationContains a 92bp insertion interrupting the open …PromoterChicken B-actinAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
J23110-mRFP1-331Bb
Plasmid#78277PurposemRFP1 behind constitutive promoter J23110 in pSEVA331Bb backboneDepositorInsertJ23110-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM4663
Plasmid#85126PurposeNSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.DepositorInsertPkaiB-luciferase
ExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-1 (GB1204)
Plasmid#75405PurposeProvides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-1 promoter
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
Plasmid#127636PurposeEncodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)DepositorInsertmTurquoiuse2-Tat, YPet-PCP
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
J23112-mRFP1-331Bb
Plasmid#78278PurposemRFP1 behind constitutive promoter J23112 in pSEVA331Bb backboneDepositorInsertJ23112-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103-mRFP1-331Bb
Plasmid#78273PurposemRFP1 behind constitutive promoter J23103 in pSEVA331Bb backboneDepositorInsertJ23103-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23105-mRFP1-331Bb
Plasmid#78275PurposemRFP1 behind constitutive promoter J23105 in pSEVA331Bb backboneDepositorInsertJ23105-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLP-mEos2-Cep123
Plasmid#72589PurposemEos2 photoconvertible fluorescent protein fused to the N-terminus of isoform 1 of Cep123 (Cep89/CCDC123)DepositorAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23101-mRFP1-331Bb
Plasmid#78272PurposemRFP1 behind constitutive promoter J23101 in pSEVA331Bb backboneDepositorInsertJ23101-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-hFX(I154F)-HA
Plasmid#14977DepositorInserthuman frataxin cDNA (FXN Human)
TagsAU1 and HAExpressionMammalianMutationchanged isoleucine 154 to phenylalanineAvailable SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACYC-mTET1-N
Plasmid#81056Purposebacterial expression of N-terminus of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationN-terminus, amino acids 301-1366PromoterT7Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a F656A
Plasmid#53054PurposeExpresses alpha subunit of F656A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Phenylalanine 656 to AlaninePromoterSP6Available SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C105A
Plasmid#122478Purposeexpresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
TagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG-C71A
Plasmid#122476Purposeexpresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
TagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codon, cysteine …Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/S1126A
Plasmid#59825PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationdelta 55/S1126APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRCIscript-crhr1_TAL1B
Plasmid#44235DepositorAvailable SinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRCIscript-crhr1_TAL1A
Plasmid#44234DepositorAvailable SinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArC K72R
Plasmid#59811PurposeAllows the integration of Myc ArC K72R in the genome and Tet-inducible expression.DepositorInsertAurora C (AURKC Human)
TagsMycExpressionMammalianMutationK72R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N748D
Plasmid#60726PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCSG49-AlleyCat7
Plasmid#42591DepositorInsertmutated C-terminal portion of calmodulin gene (M76-K148)
TagsHis and TEVExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available SinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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