We narrowed to 30,799 results for: Ide

1. INCTbiosyn-pUB-HspINTBxb1

   Plasmid

   #127519

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. OPEN HLA-A*02:01-BirA

   Plasmid

   #215569

   Purpose

   E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN HLA-A*02:01 (HLA-A Human)
   

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   Glycine 120 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. INCTbiosyn-pUB-HspINT2

   Plasmid

   #127512

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 2.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 2 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. INCTbiosyn-pUB-HspINT9

   Plasmid

   #127516

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 9.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 9 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. INCTbiosyn-pUB-HspINT13

   Plasmid

   #127517

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 13.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 13 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-CA-mDia1

   Plasmid

   #45583

   Depositor

   Ronen Zaidel-Bar, Klaus Hahn

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Insert mDia1 contains mutations V161D and N165D i…

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. OPEN-HLA-A*11:01-BirA

   Plasmid

   #235135

   Purpose

   E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*11:01 (HLA-A Human)
   

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   G120C

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. HF-PX459 (V2)

   Plasmid

   #118632

   Purpose

   Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

   Depositor

   Mike McGrew

   Article

   Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

   Insert

   hSpCas9-HF1-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Asparagine 497 to Alanine, Arginine 661 to Alanin…

   Promoter

   Cbh

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SnapTag-Binder

   Plasmid

   #179142

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-mCherry-Binder

    Plasmid

    #179139

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

    Plasmid

    #201683

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMT-Mef2c-tPT2A-GFP

    Plasmid

    #111771

    Purpose

    Bicistronic construct: Co-express two genes by 2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Insert

    Mef2c-tPT2A-GFP (Mef2c Mouse)
    

    Use

    Cloning intermediate

    Tags

    GFP

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P CISD1_1

    Plasmid

    #160773

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P CISD1_2

    Plasmid

    #160774

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. VN-Sph1

    Plasmid

    #89755

    Purpose

    expresses human Synphilin fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

    Insert

    SNCAIP (SNCAIP Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138146

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET14b His Rab8a Q67L

    Plasmid

    #186014

    Purpose

    His Rab8a Q67L

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    His Rab8a Q67L (RAB8A Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

    Plasmid

    #217759

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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