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pET-51b-HaloTag10

Plasmid

#175520

Purpose

Expression of HaloTag10 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag10

Tags

His

Expression

Bacterial

Mutation

HaloTag10 = HaloTag7-Q165W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBYK01

Plasmid

#138656

Purpose

phiOZJ integrating Streptomyces expression vector lacking RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHLM

Plasmid

#64168

Purpose

harbours marker genes HIS3, LEU2 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJMD5

Plasmid

#138652

Purpose

phiBT1 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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MOBE3

Plasmid

#219943

Purpose

Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-SL3_PT2A_apt-ABE8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-ESyn1

Plasmid

#80804

Purpose

hSyn1 promoter fused to CMVmin

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p5E-ESyn1

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pEBFP2

Plasmid

#165831

Purpose

EBFP2blue fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EBFP2

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-HaloTag-WPRE

Plasmid

#104108

Purpose

Expresses HaloTag under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

HaloTag

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFC31

Plasmid

#165845

Purpose

_C31 integrase CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 integrase

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssGFP-P2A-NLSIFP

Plasmid

#162235

Purpose

Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

GFP - IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Tags

3xFlag, His, and T7

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Hauke Hennecke, Julia Vorholt

Article

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJMD13

Plasmid

#138653

Purpose

phiC31 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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