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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMeCP2G.10 (pc1577)

Plasmid

#211728

Purpose

Expresses MeCP2 (aa1-76) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-76

Tags

GFP

Expression

Insect

Promoter

Polyhedrin promotor

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2mR (pc2519)

Plasmid

#211716

Purpose

Expresses fl. MBD2 RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

RFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD1a-C-GFP (pc1564)

Plasmid

#211711

Purpose

Expresses fl. MBD1 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD1

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PALI1_PIR_pFB1

Plasmid

#191249

Purpose

Expresses PALI1 from insect cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Tags

MBP

Expression

Insect

Mutation

NA

Promoter

Polyhedrin

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pE5771

Plasmid

#170778

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

0.4αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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PROM5_MprbcL_short_chlo

Plasmid

#165161

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL promoter region

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_Mprbcl_chlo

Plasmid

#163934

Purpose

L0 part - 5'UTR

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL 5'UTR

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_Mppetb_short_chlo

Plasmid

#163933

Purpose

L0 part - 5'UTR

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha petB 5'UTR

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3919-32

Plasmid

#176496

Purpose

pCAG-puro-T2A-TetON3G-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

puroR-T2A-TetOn3G

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSV40L-TN

Plasmid

#165827

Purpose

Viral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRs

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SV40L

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRNA2

Plasmid

#174005

Purpose

Used to generate stable mRNA for heterologous expression experiments (no polyA)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Histag_N

Plasmid

#127033

Purpose

Basic Part N- N-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBac(3xP3-gTc’v; Valcyrie.Uni)

Plasmid

#119068

Purpose

Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (low)

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
Tc-hsp68 Promoter
NLS-Cas9
Tc-hsp68 3'UTR
Tc-U6B-Uni
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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DENND1A (DENND1AA-c032)

Plasmid

#110270

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DENND1A (DENND1A Human)

Tags

TEV-His10-Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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LIMK2 (LIMK2A-c052)

Plasmid

#110264

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

LIMK2 (LIMK2 Human)

Tags

His-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-3

Plasmid

#85094

Purpose

Host vector to fuse a gene at the N-terminus of PCNA3

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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TAL-BBL1-ID14

Plasmid

#41516

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

Polylinker flanked by 2 Mva1269I cut sites

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TAL-BBL1-ID43

Plasmid

#41517

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

Polylinker flanked by 2 Mva1269I cut sites

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TAL-BBL1-ID32

Plasmid

#41518

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

Polylinker flanked by 2 Mva1269I cut sites

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TAL-BBL1-ID21

Plasmid

#41519

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

Polylinker flanked by 2 Mva1269I cut sites

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBACgus N-His/GST/LRRK2 1867-2141

Plasmid

#40404

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2141

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus N-His/GST/LRRK2 1867-2161

Plasmid

#40405

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2161

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus4x/cdc37/LRRK2 1867-2186_C2024S/C2025S

Plasmid

#40366

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

aa1867-2186, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40372

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

aa1867-2161, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S

Plasmid

#40388

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pCY 3120-01

Plasmid

#36237

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yECitrine-7Myc

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-7Myc-kanMX

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pOX + JShal XR

Plasmid

#16148

Depositor

Larry Salkoff

Article

Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

Insert

Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pLIB-RSP1

Plasmid

#176690

Purpose

Expression of Chlamydomonas reinhardtii RSP1 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP1

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP2

Plasmid

#176691

Purpose

Expression of Chlamydomonas reinhardtii RSP2 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP2

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP9

Plasmid

#176697

Purpose

Expression of Chlamydomonas reinhardtii RSP9 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP9

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP10

Plasmid

#176698

Purpose

Expression of Chlamydomonas reinhardtii RSP10recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP10

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge

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