We narrowed to 27,863 results for: sta

1. pSAVED-CHAT_PCaspase_PCi

   Plasmid

   #214049

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-PCi
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS-BCD-Strep-PC-σ

   Plasmid

   #214037

   Purpose

   Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PC-σ
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Tau Authentic Alu 10-12WT

   Plasmid

   #194167

   Purpose

   Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT
   

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_Tau Authentic Alu 7-12WT

   Plasmid

   #194170

   Purpose

   Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   microtubule-associated protein tau 7-12 WT
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ZKSCAN1_Tau10-12V337M

   Plasmid

   #194165

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 V337M
   

   Expression

   Mammalian

   Mutation

   Changed Valine 337 to Methinonine

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2z-lmo2

   Plasmid

   #164657

   Purpose

   for the synthesis of zebrafish lmo2 mRNA

   Depositor

   Didier Stainier

   Article

   Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

   Insert

   lmo2 (lmo2 Zebrafish)
   

   Use

   Mrna synthesis

   Promoter

   SP6

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3020_pETcon-SARS-CoV-2-RBD_N501Y

   Plasmid

   #184405

   Purpose

   yeast surface display of the SARS-CoV-2 Alpha variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   N501Y

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458_sgAgo2_Nterm

   Plasmid

   #170920

   Purpose

   Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo2 (Ago2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458_sgAgo1_Nterm

   Plasmid

   #170921

   Purpose

   Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1 (Ago1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO-BcRF1-ORF24-2xStrep

    Plasmid

    #138454

    Purpose

    Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    BcRF1 amino acids 1-178 fused to ORF24 amino acid…

    Promoter

    CMV

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO-UL87-ORF24-2xStrep

    Plasmid

    #138455

    Purpose

    Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87-ORF24 (UL87, ORF24 HCMV/KSHV)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    UL87 amino acids 1-248 fused to ORF24 amino acids…

    Promoter

    CMV

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-SENP2 NPm

    Plasmid

    #126593

    Purpose

    Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-65 and L329A/L331A in t…

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLAP-BUBR1-3A

    Plasmid

    #114054

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-3A (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLAP-BUBR1-3D

    Plasmid

    #114055

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-3D (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmirGlo-CALB2-3'UTR mir-30 mut1

    Plasmid

    #74428

    Purpose

    Calb2 UTR with mutated mir-30 site 1

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. xCIAR_pcDNA5/FRT/TO

    Plasmid

    #86504

    Purpose

    Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    xCIAR (SOS1 Human)
    

    Use

    Frt

    Expression

    Mammalian

    Mutation

    F929A and T968L in SOScat

    Promoter

    CMV (tet operator)

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmirGlo-CALB2-3'UTR delta ARE

    Plasmid

    #74425

    Purpose

    Calb2 UTR with mutated AU binding sequence

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid

    #229498

    Purpose

    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC 5'Untranslated region + destabilized GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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