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EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

Plasmid

#42185

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Gamma 3(F70A, F71A) (Gng3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F70A, F71A

Promoter

cmv

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

Plasmid

#42188

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Gamma 3(K68A,K69A) (Gng3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

K68A,K69A

Promoter

cmv

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-7th R

Plasmid

#42573

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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alpha 0-CFP in pdest12.2

Plasmid

#36312

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-MED20

Plasmid

#15435

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED20 (Med20 Mouse)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Expression

Worm

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Article

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 1

Plasmid

#42543

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-MYB

Plasmid

#51305

Purpose

Expression of MYB after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-myb avian myeloblastosis viral oncogene homolog (MYB Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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GANLS-DEVD-BNES

Plasmid

#50835

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddRFP B

Tags

NES sequence LALKLAGLDIGS and triplicated NLS seq…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-IKKbeta (S177E,S181E)-pcw107

Plasmid

#64609

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

IKBKB

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-FLEX-ArchT-tdTomato

Plasmid

#28305

Purpose

Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV5

Insert

ArchT-tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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shTCF7L2 # 1

Plasmid

#42557

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only