We narrowed to 5,974 results for: SPI
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6691 Bicistronic_ires_puro
Plasmid#64335PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance geneDepositorTypeEmpty backboneUseRetroviralAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAHS2 (pBS0249)
Plasmid#185139PurposeFor the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertCAHS2
ExpressionMammalianAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralPromoterUBCAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
rnLbCas12a-PE2
Plasmid#213746PurposeFor circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cellsDepositorInsertrnLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, H759A, R1138A in LbCas12aPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET 2C-T10-dCasX
Plasmid#126418PurposeE. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purificationDepositorInsertDpbCasX
UseCRISPRTagsHis-MBPExpressionBacterialAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph20
Plasmid#133024PurposeExpresses Xph20 in bacteriaDepositorInsertXph20
TagsHis10ExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag-SIRT1 H355A
Plasmid#8439DepositorInsertSIRT1 (Sirt1 Mouse)
UseAdenoviralTagsFlagExpressionMammalianMutationHistidine 355 mutated to AlanineAvailable SinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHS638_Flex-Cas12a-CBE
Plasmid#244845PurposeMammalian expression of Flex-Cas12a cytosine base editorDepositorInsertFlex-dCas12a-CBE
UseCRISPRTags2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…ExpressionMammalianMutationG146R/R182V/D535G/S551F/D665N/E795Q/D832APromoterCMVAvailable SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
L2092-GPA-FullBRET-Retrovirus-TD142
Plasmid#222877PurposeRetroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.DepositorInsertFRB-GPA-NanoLuc-CyOFP1
UseRetroviralTagsMycExpressionMammalianPromoterMSCV LTRAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP EGFP-ER [TM(SAC1)]
Plasmid#186475PurposeExpression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cellsDepositorInsertSAC1 (SACM1L Human)
UseRetroviralTagsEGFPExpressionMammalianMutationC-terminal transmembrane region of the protein: 5…Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248AAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST15 hSlp4-a
Plasmid#40046DepositorAvailable SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
lentiviral shRNA GEF-H1
Plasmid#21477DepositorInsertrho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
UseLentiviralTagseGFPExpressionMammalianAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUGP2
Plasmid#211531PurposeExpresses codon optimized UGP2DepositorAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLO ddd64.1
Plasmid#123121PurposeE. coli expression vector for catalytically inactivated deltaproteobacteria CasXDepositorInsertCasX
UseCRISPRMutationMutated amino acids in catalytic triad to alanine…Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only
