We narrowed to 10,059 results for: Uty

1. pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

   Plasmid

   #191635

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MSP1D1deltaH4/2H5

   Plasmid

   #71715

   Purpose

   Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p164 Dync1i1.B

   Plasmid

   #26435

   Depositor

   Elizabeth Fisher

   Article

   Kuta et al PLoS One. 2010 . 5(7):e11682.

   Insert

   cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
   

   Use

   Subcloning and sequencing

   Mutation

   N/A

   Available Since

   March 21, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pEF Doc2alpha WT GFP

   Plasmid

   #128819

   Purpose

   To visualize Doc2alpha WT in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. IKK2-EYFP

   Plasmid

   #111196

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   IKKbeta (IKBKB Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

   Plasmid

   #105040

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLINTR BFP v1 backbone

   Plasmid

   #179779

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

   Plasmid

   #40806

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(48-55) + S222D + S226D

   Promoter

   MSV-LTR

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX302-MKP7-V5 puro

    Plasmid

    #87771

    Purpose

    Expresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAVA-LGF2-Gal11p

    Plasmid

    #127481

    Purpose

    Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2-Gal11p (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSW Doc2alpha WT

    Plasmid

    #128812

    Purpose

    To make virus expressing Doc2alpha WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFSW Doc2beta WT

    Plasmid

    #128814

    Purpose

    To make virus expressing Doc2beta WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJA104

    Plasmid

    #128520

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme I56T (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-GTSE1-His6

    Plasmid

    #69109

    Purpose

    For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    G2- and S-phase Expressed 1 (GTSE1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEBac1-beta-actin

    Plasmid

    #178076

    Purpose

    Insect cell expression of beta-actin

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    beta-actin (ACTB Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAVA-Gal11p-LGF2

    Plasmid

    #127480

    Purpose

    Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2 (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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