We narrowed to 13,280 results for: cDNA

1. pcDNA3.1-Cerulean-Epac2-Venus

   Plasmid

   #138196

   Purpose

   FRET-based biosensor for monitoring Epac2 activation.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-FLAG-Dicer-delPAZ

   Plasmid

   #51367

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to PAZ domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/NDUFS7/myc-His

   Plasmid

   #127488

   Purpose

   Expresses myc-tagged NDUFS7 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS7 (NDUFS7 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TOgw-C-mCherry

   Plasmid

   #223952

   Purpose

   Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FLAG-Dicer-delDEAD

   Plasmid

   #51364

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to DEAD domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-3xFLAG-NLS-TPP1

   Plasmid

   #53585

   Purpose

   Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Silent mutation to eliminate PacI site

   Promoter

   CMV

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-N-Myc_535-888 USP7

    Plasmid

    #131248

    Purpose

    Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid

    #131249

    Purpose

    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

    Plasmid

    #127404

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT EGFP-miniAID

    Plasmid

    #101714

    Purpose

    For amplification of a EGFP-miniAID for N-terminal tagging

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    EGFP-miniAID
    

    Use

    Tags

    EGFP-miniAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-GFP-EphrinB3-Flag

    Plasmid

    #155012

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-FLAG-Dicer-delDUF

    Plasmid

    #51365

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to DUF domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-N-Flag-8His

    Plasmid

    #157677

    Purpose

    N-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Flag-8His
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-FLAG-MTOR-V2006I

    Plasmid

    #69012

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V2006I

    Promoter

    CMV

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CD81-(TEV)-Cre

    Plasmid

    #167939

    Purpose

    human CD81-TEV cleavage site-Cre

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    Cre recombinase and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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