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pKDC.154

Plasmid

#222228

Purpose

Acceptor plasmid for human retron-Eco1 variants.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Type

Empty backbone

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pNano

Plasmid

#169895

Purpose

Minimal cloning vector

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Cloning

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-G

Plasmid

#137090

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CylLS_CylM

Plasmid

#208760

Purpose

Expresses modified cytolysin S (mCylLS) in E. coli

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLS
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CD55-full length

Plasmid

#220874

Purpose

Expresses CD55 variant in mammalian cells

Depositor

Ferenc Scheeren

Insert

CD55 (CD55 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMW

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD002

Plasmid

#48834

Purpose

Provides a default dummy as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

D-Dummy

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::GW,attB}

Plasmid

#182171

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMal-E4rGel

Plasmid

#51280

Purpose

Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

Insert

E4rGel

Expression

Bacterial

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pL2-G(P)7G

Plasmid

#137100

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(P)7G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G2

Plasmid

#137091

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GG

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G3

Plasmid

#137092

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pFD-FKBP12

Plasmid

#137108

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FKBP12

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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