Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 2,347 results for: puc19

Showing: 1051 - 1100 of 2347 results

  22. pE2F5-donor

    Plasmid
    #112351
    Purpose
    CRISPR donor plasmid to tag human transcription factor E2F5 with GFP
    Depositor
    Kevin White
    Insert
    E2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:85213859:T>C, hg38:8:85213860:T>G, h…
    Promoter
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112351-plasmid-map-sequence-id-229665

    Enlarge

  23. pZNF354B-donor

    Plasmid
    #112309
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP
    Depositor
    Kevin White
    Insert
    ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:5:178884427:G>A, hg38:5:178883445:C>T,…
    Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112309-plasmid-map-sequence-id-228461

    Enlarge

  24. pAW8_3HA

    Plasmid
    #110233
    Purpose
    Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110233-plasmid-map-sequence-id-219788

    Enlarge

  36. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

    Enlarge

  39. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

    Enlarge

  41. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

    Enlarge

  43. pG108 - K

    Plasmid
    #178039
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers
    Depositor
    Janina Lewis
    Article
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Insert
    Kanamycin resistance
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ErmAM has been replaced with Kanamycin for select…
    Promoter
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    178039-plasmid-map-sequence-id-378887

    Enlarge

  44. pDAC569

    Plasmid
    #195241
    Purpose
    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195241-plasmid-map-sequence-id-384464

    Enlarge

  49. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1051 - 1100 of 2347 results