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pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA065

Plasmid

#128517

Purpose

Expresses Ber e 1 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

Ber e 1 a allergenic 2S albumin from Brazil nut

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM015

Plasmid

#128549

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 5

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM013

Plasmid

#128548

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 4

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM010

Plasmid

#128546

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 2

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM012

Plasmid

#128547

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 3

Expression

Yeast

Promoter

AOX1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGJM09

Plasmid

#128545

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 1

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5a

Plasmid

#221439

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5a (Fam46a Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMJA181

Plasmid

#128539

Purpose

Expresses anti-Prunus dulcis scFv Ab+BirA tag

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6 plus BirA tag

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c

Plasmid

#221440

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMJA219= pcDNA3.1V5-completeTRABV-CMVp

Plasmid

#116878

Purpose

mammalian expression vector containing TCR sequences

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)

Expression

Mammalian

Promoter

CMV Promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c_dCAT

Plasmid

#221442

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c_AA (dCAT) (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Mutation

D90A, D92A (GATctgGAT to GCCctgGCC)

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

Plasmid

#116876

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA089

Plasmid

#128518

Purpose

Expresses human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA167

Plasmid

#128536

Purpose

Expresses DsRed

Depositor

Marcos Alcocer

Article

Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

Insert

NFAT-DsRed reporter

Expression

Mammalian

Promoter

SV40-NFAT

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA285= pSin-TCRab-CMVp-Puro

Plasmid

#116875

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TCR (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of EF1-a promotor

Promoter

CMV Promotor

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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