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Plasmid#218904Purposegolden gate cloningDepositorInserthPGK-PuroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)
Plasmid#172328PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-hKLF4
Plasmid#60435PurposeOver-expression of human KLF4DepositorInsertKLF4 (KLF4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGK1 gRNA (BRDN0001144730)
Plasmid#77980Purpose3rd generation lentiviral gRNA plasmid targeting human PGK1DepositorInsertPGK1 (PGK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGK1 gRNA (BRDN0001487049)
Plasmid#77981Purpose3rd generation lentiviral gRNA plasmid targeting human PGK1DepositorInsertPGK1 (PGK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-Chst3
Plasmid#193024PurposeAAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInsertChst3 (Chst3 Mouse)
UseAAVTags3xFLAG and myc-tagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::tTA2-bGHpA
Plasmid#62448PurposeMXS_chaining vector with PGK::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with PGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGK1 gRNA (BRDN0001146969)
Plasmid#77982Purpose3rd generation lentiviral gRNA plasmid targeting human PGK1DepositorInsertPGK1 (PGK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6 (-cyt)
Plasmid#137817Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region, R296K (please see dep…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 2, G318A
Plasmid#188365PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE:shRNA A-pGK:rtTA-Cre
Plasmid#68471PurposeInducible lentivirus expressing an arbitrary shRNA and CreDepositorInsertsGFP-shRNA A-miR30
rtTA-2A-Cre
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterpGK and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE.IkBSR-Flag Pgk.Cre
Plasmid#22504DepositorInsertIkBSR
UseCre/Lox and LentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-rtetTAK8N, L131L
Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::HygroR-bGHpA
Plasmid#62436PurposeMXS_chaining vector with PGK::HygroR-bGHpADepositorInsertresistance cassette against hygromycin B with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::rtTA3-bGHpA
Plasmid#62446PurposeMXS_chaining vector with PGK::rtTA3-bGHpADepositorInsertcassette containing the reverse tetracycline transactivator with PGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W
Plasmid#163169PurposeLentiviral plasmid with non-targeting gRNA, co-expression of BFP tagDepositorInsertNon-targeting
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44s (-cyt)
Plasmid#137815Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44s (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only