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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 144,362 results for: ins

Showing: 421 - 440 of 144362 results

  4. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

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  7. kanMX6-ins1

    Plasmid
    #195038
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195038-plasmid-map-sequence-id-384240

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  10. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

    Plasmid
    #173213
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173213-plasmid-map-sequence-id-339522

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  17. 36INS2-pBAD_GB

    Plasmid
    #78677
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    78677-plasmid-map-sequence-id-147244

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  18. 36INS2-pBAD_FB

    Plasmid
    #78676
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78676-plasmid-map-sequence-id-147245

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  19. 36INS2-pBAD_EB

    Plasmid
    #78675
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78675-plasmid-map-sequence-id-147246

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  20. 36INS2-pBAD_AB

    Plasmid
    #78674
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78674-plasmid-map-sequence-id-147247

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Showing: 421 - 440 of 144362 results