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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 157,699 results for: ins

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Showing: 421 - 440 of 157699 results

  1. proinsulin-mGold

    Plasmid
    #218953
    Purpose
    Expresses proinsulin fused to mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-mGold
    Tags
    proinsulin
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218953-plasmid-map-sequence-id-446158

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  2. his3MX6-ins3

    Plasmid
    #195040
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    His5+
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195040-plasmid-map-sequence-id-384243

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  4. CAPZB2-TwinStrepII

    Plasmid
    #83194
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZB2 (Capzb Mouse)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83194-plasmid-map-sequence-id-163493

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  5. natMX-ins7

    Plasmid
    #195044
    Purpose
    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195044-plasmid-map-sequence-id-384248

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  7. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

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  10. pIS1 INSIG2

    Plasmid
    #60798
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60798-plasmid-map-sequence-id-97809

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  13. pIS1 mINSIG2

    Plasmid
    #60799
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    INSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60799-plasmid-map-sequence-id-112562

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  19. proinsulin-msYFP2

    Plasmid
    #218955
    Purpose
    Expresses proinsulin fused to msYFP2 in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-msYFP2
    Tags
    proinsulin
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218955-plasmid-map-sequence-id-446161

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  20. GBH-R-insBFP.2

    Plasmid
    #188008
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; insulin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188008-plasmid-map-sequence-id-381105

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Showing: 421 - 440 of 157699 results