167,785 results

1. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV9)

   Viral Prep

   #100836-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   CAG

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

3. AAV-FLEX-Arch-GFP (AAV9)

   Viral Prep

   #22222-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Promoter

   CAG

   Tags

   GFP (Cre-dependent)

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pX330A-1x2

   Plasmid

   #58766

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-NbALFA

   Plasmid

   #159987

   Purpose

   For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mCherry-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mCherry and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MoClo CRISPR/Cas Toolkit for Plants

   Plasmid Kit

   #1000000159

   Purpose

   The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.

   Depositor

   Vladimir Nekrasov

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-FAH-rtTA3G

   Plasmid

   #120309

   Purpose

   An AAV vector that expresses rtTA3G under the c-fos promoter

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   rtTA3G
   

   Use

   AAV

   Promoter

   c-fos

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL1 sortase

    Plasmid

    #106288

    Purpose

    Expresses sortase in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    sortase A pentamutant
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-mt-mKeima-P2A-FRB-Fis1

    Plasmid

    #135295

    Purpose

    stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (AAV5)

    Viral Prep

    #130998-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    EF1a

    Tags

    mScarlet

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

13. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-cFos-tTA-pA

    Plasmid

    #66794

    Purpose

    Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

    Depositor

    William Wisden

    Article

    Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

    Insert

    cfos promoter linked to the tet activator gene
    

    Use

    AAV

    Tags

    no

    Expression

    Mammalian

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid

    #181751

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV SYN PSAM4 GlyR IRES EGFP (AAV5)

    Viral Prep

    #119742-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV SYN PSAM4 GlyR IRES EGFP (#119742). In addition to the viral particles, you will also receive purified AAV SYN PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only