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Plasmid Kit#1000000023PurposeLIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semiâautomated TALE generationDepositorAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hDlx-Flex-GFP-Fishell_6 (AAV Retrograde)
Viral Prep#83895-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. mDlx-driven, Cre-dependent expression of GFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsGFPAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.P301L-Tau
Plasmid#140425PurposeAAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTauDepositorInserteGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
UseAAVTagseGFPExpressionMutationchanged Proline 301 to LeucinePromoterCBAAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CFP
Plasmid#13030PurposeMammalian expression vector for cloning your gene fused to CFPDepositorHas ServiceCloning Grade DNAInsertcyan fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 T56N
Plasmid#86789Purposemammalian expression of Rab12 T56N, constitutively GDP-bound mutantDepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationT56N, constitutively GDP-bound mutantPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Stat5
Plasmid#139832Purposedoxycycline-inducible expression of mouse Stat5 in mammalian cellsDepositorInsertStat5 (Stat5a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (AAV PHP.eB)
Viral Prep#214858-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (#214858). In addition to the viral particles, you will also receive purified AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) plasmid DNA. minBG-driven expression of SYFP2 plus a separate nuclear FLAG epitope in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsSYFP2Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS2
Plasmid#218712PurposeExpresses N-terminally EGFP-tagged BBS2 in mammalian cellsDepositorInsertBBS2 (BBS2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV1)
Viral Prep#162380-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10
Plasmid#24749DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationwildtype sequencePromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-6a-Ataxin3Q22
Plasmid#22126DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-hESRRB
Plasmid#60434PurposeOver-expression of human ESRRBDepositorInsertESRRB (ESRRB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry
Plasmid#182377PurposeDual expression construct encoding 3xFLAG and mCherry from separate promotersDepositorInserts3xFlag
mCherry
UseTagsExpressionMammalianMutationPromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-MCS-Ds
Plasmid#102416PurposeEmpty vector with a multiple cloning site flanked by Ds integration elementsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP
Plasmid#220797PurposeshRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neuronsDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a8
Plasmid#124848PurposeMutagenesis of Slc17a8DepositorInsertSlc17a8 (Slc17a8 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoCDA1
Plasmid#195515PurposeUbiquitous expression of dCBEevoCDA1 in DrosophilaDepositorInsertdCBE-evoCDA1
UseCRISPRTagsExpressionInsectMutationPromoteract5CAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-wildtype
Plasmid#179684PurposeExpression of base editor in mammalian cellsDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only