We narrowed to 34,877 results for: cas

1. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

   Plasmid

   #117324

   Purpose

   CRISPR-Cas9 for miR-124-1, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1-3' (MIR124-1 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

   Plasmid

   #117323

   Purpose

   CRISPR-Cas9 for miR-124-3, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-3-5' (MIR124-3 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

   Plasmid

   #117322

   Purpose

   CRISPR-Cas9 for miR-124-1/2, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mei-S332 5.6Kb genomic DNA in Casper4

   Plasmid

   #112984

   Purpose

   P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

   Insert

   mei-S332 (Sgo) (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK4387 - human expression plasmid for SpCas9 Cluster 5

   Plasmid

   #101191

   Purpose

   Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster5(K918A/V922A/R925A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K918A/V922A/R925A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMW3645 - human expression plasmid for SpCas9 Cluster 3

   Plasmid

   #101187

   Purpose

   Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   T657A/G658A/W659A/R661A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMW3770 - human expression plasmid for SpCas9 Cluster 4

   Plasmid

   #101189

   Purpose

   Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   F491A/M495A/T496A/N497A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

   Plasmid

   #91884

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

10. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

    Plasmid

    #91877

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

11. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

    Plasmid

    #91883

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid

    #87148

    Purpose

    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-zwf guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid

    #87154

    Purpose

    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

    Plasmid

    #69221

    Purpose

    Expresses R1333K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

    Plasmid

    #69222

    Purpose

    Expresses R1333S mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Serine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hy_pMT Laccase2 MCS-ZKSCAN1 548-847

    Plasmid

    #69885

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiFUCCI(CA)5

    Plasmid

    #223176

    Purpose

    Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

    Depositor

    Neville Sanjana

    Article

    Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

    Insert

    FUCCI(CA)5
    

    Use

    Lentiviral

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tFucci(CA)5pPBbsr2

    Plasmid

    #197351

    Purpose

    Encoding tFucci(CA)5.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    tFucci(CA)5
    

    Use

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-CA-mDia1

    Plasmid

    #45583

    Depositor

    Ronen Zaidel-Bar, Klaus Hahn

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Insert mDia1 contains mutations V161D and N165D i…

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only