We narrowed to 30,799 results for: Ide

1. pSCrhaB2_ApyOAHIDS

   Plasmid

   #226246

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   ApyS
   

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCrhaB2_ApyOAHID

   Plasmid

   #226245

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG071

   Plasmid

   #214257

   Purpose

   Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
   

   Expression

   Bacterial

   Mutation

   Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

   Promoter

   araBAD promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG075

   Plasmid

   #214258

   Purpose

   Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M
   

   Expression

   Bacterial

   Mutation

   Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

   Promoter

   araBAD promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28:LanI-101

   Plasmid

   #225065

   Purpose

   Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanI-101)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBS-839

   Plasmid

   #217995

   Purpose

   sAP1 protein secretion signal

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

   Plasmid

   #206464

   Purpose

   Oskar 139-240 mut expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Oskar 139-240 mut (osk Fly)
   

   Tags

   PH mcherry

   Expression

   Insect

   Mutation

   A162E L228E)

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

   Plasmid

   #220494

   Purpose

   To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting LIG4 exon 3 (LIG4 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

    Plasmid

    #217760

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid

    #216116

    Purpose

    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-mini v2-HF24_F4

    Plasmid

    #195718

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#4 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 20, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pUC57-mini v2-HF24_F20

    Plasmid

    #195734

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#20 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pUC57-mini v2-HF24_F5

    Plasmid

    #195719

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#5 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pUC57-mini v2-HF24_F12

    Plasmid

    #195726

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#12 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUC57-mini v2-HF24_F3

    Plasmid

    #195717

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#3 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pUC57-mini v2-HF24_F24

    Plasmid

    #195738

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#24 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pUC57-mini v2-HF24_F7

    Plasmid

    #195721

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#7 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUC57-mini v2-HF24_F8

    Plasmid

    #195722

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#8 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pUC57-mini v2-HF24_F14

    Plasmid

    #195728

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#14 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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