We narrowed to 5,068 results for: cre encoding plasmid

1. AAV-Synapsin-mScarlet-optoJNKi-WPRE

   Plasmid

   #159968

   Purpose

   optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mScarlet-optoJNKi
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-Synapsin-Ypet-optoJNKi-WPRE

   Plasmid

   #159967

   Purpose

   optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   Ypet-optoJNKi
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCASP-SptP92-TD-HilA

   Plasmid

   #153331

   Purpose

   Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP92-HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Use

   Tags

   Flag tag with linker and mitochondrial targeting …

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMMP-dnPERK-IRES-eGFP

   Plasmid

   #36954

   Depositor

   Bill Sugden

   Article

   Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

   Insert

   dnPERK (EIF2AK3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deletion of aa592-1061 to create dominant negative

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPT-/GCCACC-mEGFP-IRES-mCherry

   Plasmid

   #49235

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPT-/CGA-mEGFP-IRES-mCherry

   Plasmid

   #49233

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3A9cbetaTCR.20beta

   Plasmid

   #69592

   Purpose

   TCR beta chain genomic transgenic construct for creating OT-II mice

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR beta VJ region from clone 1.1 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pWPT-ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49229

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49234

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49228

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49232

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWPT-CCC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49231

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWPT-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49230

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid

    #194328

    Purpose

    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

    Depositor

    Alexander McLellan

    Article

    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Tet-responsive promotor related mutations: G72V i…

    Promoter

    Tight tet-responsive promotor

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234437

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234438

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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