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EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 wt

Plasmid

#80876

Purpose

mammalian expression of ALK5 WT

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

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syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-NFAT luciferase

Plasmid

#17870

Purpose

3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

3x NFAT binding sequence (IL2 )

Use

Luciferase

Tags

IL2 promoter and Luciferase

Expression

Mammalian

Promoter

IL-2 promoter

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-TurboID-NES_pLX304

Plasmid

#202008

Purpose

expresses TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-TurboID-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-EGFP

Plasmid

#80945

Purpose

human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

EGFP

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1 Catalytic Inactive

Plasmid

#237796

Purpose

Inducible lentiviral expression of RNaseH1 Catalytic Inactive

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Expression

Mammalian

Mutation

D210N

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTubulin-Electra2

Plasmid

#184930

Purpose

Tubulin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFYF1320 EGFP Site#1

Plasmid

#47511

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_mCherry

Plasmid

#182939

Purpose

alpha7 nACHR expression in mammalian cell lines

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Use

Synthesized

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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