We narrowed to 18,840 results for: EMB

1. W118-1_hZBTB7B_R363L

   Plasmid

   #180381

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R363L (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   R363L

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. W118-1_hZBTB7B_D35N_R49Q

   Plasmid

   #180383

   Purpose

   lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_D35N/R49Q (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D35N/R49Q

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_hZBTB7B_R49H

   Plasmid

   #180382

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R49H (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   R49H

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

   Plasmid

   #148440

   Purpose

   Mammalian Expression of HsAGO2

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsAGO2 (AGO2 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation C840Tand sequence differs in …

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b MOSPD2 [315-445]

   Plasmid

   #186474

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [315-445]

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBpuro-EGFP-eDHFR(69K6)-cRaf

   Plasmid

   #178849

   Purpose

   Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET-22-DExCon-mNeonGreen-R11a

   Plasmid

   #179897

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

   Plasmid

   #148420

   Purpose

   Mammalian Expression of HsRck_296-472-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck_296-472-10xmut (DDX6 Human)
   

   Expression

   Mammalian

   Mutation

   Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV.CBA.YFP.miR-E_shRps6ka2-3

    Plasmid

    #180390

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-3 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV.CBA.YFP.miR-E_shRps6ka2-2

    Plasmid

    #180389

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-2 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.YFP.miR-E_shRps6ka2-1

    Plasmid

    #180388

    Purpose

    Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shRps6ka2-1 (Rps6ka2 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid

    #145766

    Purpose

    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

    Depositor

    Marcel Bruchez

    Article

    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

    Insert

    kappa-myc-dK-2xG4S-TMst
    

    Expression

    Mammalian

    Mutation

    The dK FAP is dL5 with mutations at E50K, L89S, a…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-TEV-mAmber

    Plasmid

    #105792

    Purpose

    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mAmber

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-mAmber-TEV

    Plasmid

    #105775

    Purpose

    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mAmber-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 10xHis-ataxin-3 *SIM-HA

    Plasmid

    #89983

    Purpose

    Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Tags

    10xHis and HA

    Expression

    Mammalian

    Mutation

    mutated aa 162IFVV to 162AFAA

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ataxin-3 *SIM

    Plasmid

    #89979

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 162IFVV to 162AFAA

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NKP9 pmRFP-N2 (719)

    Plasmid

    #61999

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    C17orf62 (CYBC1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. NKP40 RFP (794)

    Plasmid

    #62006

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    C17orf32 (C17orf32 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTH731-2µ-RLuc/minCFLuc

    Plasmid

    #40601

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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