We narrowed to 14,084 results for: cas9

1. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

   Plasmid

   #79382

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS280

   Plasmid

   #73738

   Purpose

   Simple cloning vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV_AA100

   Plasmid

   #245322

   Purpose

   Cas9 CRISPRko all-in-one positive control; targets CD55

   Depositor

   John Doench, David Root

   Insert

   Cas9, sgRNA CD55 (CD55 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2_PROX1_1+4

   Plasmid

   #121177

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZA31-sulA-GFP

   Plasmid

   #78493

   Purpose

   Expression of GFP under the sulA promoter.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Insert

   GFPmut2
   

   Expression

   Bacterial

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE2-P2A-PuroR

   Plasmid

   #196962

   Purpose

   Expresses PE2 with puromycin resistance

   Depositor

   Leopold Parts

   Insert

   PE2-P2A-Puro
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSA_0_mKate2_synCoTC

    Plasmid

    #199469

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSA_2_mKate2_synCoTC

    Plasmid

    #199471

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSA_2_mTagBFP2_synCoTC

    Plasmid

    #199474

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSA_0_mTagBFP2_synCoTC

    Plasmid

    #199472

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSA_1_mKate2_synCoTC

    Plasmid

    #199470

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSA_1_mTagBFP2_synCoTC

    Plasmid

    #199473

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLC13

    Plasmid

    #78494

    Purpose

    Expression of Mu-gam under the pBAD promoter

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    Mu-gam (Mup10 )
    

    Expression

    Bacterial

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB30

    Plasmid

    #111910

    Purpose

    Expresses gRNA to target URA3 knockout locus and has KanMX marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting URA3 knockout locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKozak_mTagBFP2_synCoTC

    Plasmid

    #199493

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid

    #107306

    Purpose

    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    TALE_VEGFA-TS3
    

    Use

    CRISPR

    Tags

    3x Flag, 2xNLS and FRB

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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