We narrowed to 9,669 results for: construct

1. pAJ12

   Plasmid

   #14914

   Depositor

   James Lok

   Article

   Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

   Insert

   multiple cloning site 3
   

   Use

   Tags

   GFP(S65C)

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJ14

   Plasmid

   #14916

   Depositor

   James Lok

   Article

   Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

   Insert

   multiple cloning site 3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAJ15

   Plasmid

   #14917

   Depositor

   James Lok

   Article

   Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

   Insert

   multiple cloning site 3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSET FLII282Pglu-4m

   Plasmid

   #13564

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A T282S-ECFP-N283R
   

   Use

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCKTRBS-CbnR-ABE44898-OD

   Plasmid

   #102925

   Purpose

   pCKTRBS-OD derivative carrying CbnR-ABE44898-OD chimera, also known as ChBz01

   Depositor

   George Church

   Article

   Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

   Insert

   CbnR-ABE44898-OD (ChTFBz01)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PtetO

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3+ hSlo2.2

   Plasmid

   #16214

   Depositor

   Larry Salkoff

   Insert

   KCNT1 potassium channel, subfamily (KCNT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   This construct lacks the first 53aa when compared…

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOX + hSlo2.2

   Plasmid

   #16215

   Depositor

   Larry Salkoff

   Insert

   KCNT1 potassium channel, subfamily (KCNT1 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   This construct lacks the first 53aa when compared…

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

9. BW-Para

   Bacterial Strain

   #172602

   Purpose

   The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

   Depositor

   Liskin Swint-Kruse

   Article

   Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

   Bacterial Resistance

   None

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

10. pCE27

    Plasmid

    #174405

    Purpose

    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCE35

    Plasmid

    #174409

    Purpose

    C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. R26TV CAG LSL 2-5

    Plasmid

    #68412

    Purpose

    Rosa26 targeting vector backbone for use with GMAP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox and Mouse Targeting; Gmap

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-FRT-KanR-FRT pA

    Plasmid

    #82601

    Purpose

    3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV Puro-CMV:GFP

    Plasmid

    #68485

    Purpose

    Retrovirus expressing GFP driven by CMV promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCGN N-Ras wt

    Plasmid

    #14723

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    N-ras (NRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRosa26 R1-ccdB-R2 RexNeo PI-SceI

    Plasmid

    #24418

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV/3' Box_(GLuc)_TOP1

    Plasmid

    #68434

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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