We narrowed to 27,863 results for: sta

1. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

   Plasmid

   #140196

   Purpose

   Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dSpCas9-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta) , NLS

   Promoter

   PgpdA and PtrpC

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3086

   Plasmid

   #144562

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Puro DEST (w118-1) eGFP

   Plasmid

   #107505

   Purpose

   Expresses eGFP, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-mOrange

   Plasmid

   #102632

   Purpose

   In vitro expression of mOrange from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mOrange
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-YPet

   Plasmid

   #102633

   Purpose

   In vitro expression of YPet from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   YPet
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-SLC51A_STOP

   Plasmid

   #161406

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC51A (SLC51A Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJL1-mTFP1

   Plasmid

   #102635

   Purpose

   In vitro expression of mTFP1 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTFP1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-PA-Rac1-I539E

   Plasmid

   #22026

   Purpose

   Expression of photoactivatable Rac1 ('lit' state mutant)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
   

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains 'lit state' mutatio…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSBbi-RP-CoV2/stSpike-puro

   Plasmid

   #161794

   Purpose

   Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

   Insert

   SARS-CoV-2 secretable trimeric Spike
   

   Use

   Transposon

   Tags

   6xHis and dTomato

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SOX2-P2A-tagBFP-HDR

    Plasmid

    #163751

    Purpose

    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    P2A-tagBFP

    Mutation

    Does not contain start codon to avoid random inte…

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX LYCHOS (GPR155) WT-FLAG

    Plasmid

    #199633

    Purpose

    Lentiviral construct to stably express LYCHOS (GPR155) WT

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1-mKalama1

    Plasmid

    #102639

    Purpose

    In vitro expression of mKalama1 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    mKalama1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid

    #91781

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Asp (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1-CyPet

    Plasmid

    #102636

    Purpose

    In vitro expression of CyPet from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    CyPet
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

    Plasmid

    #177144

    Purpose

    Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid

    #91733

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX LYCHOS-FLAG-TiD

    Plasmid

    #199747

    Purpose

    Lentiviral construct to stably express LYCHOS (GPR155) TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pChuk

    Plasmid

    #87033

    Purpose

    express IKKalpha in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    IKKalpha (Chuk Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV Puro DEST (w118-1) FITM2

    Plasmid

    #107504

    Purpose

    Expresses FITM2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FITM2 (codon optimized)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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