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pLIGFP_W220F

Plasmid

#46772

Purpose

Protein overexpression with the modified LacIWF repressor

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI_W220F (lacI )

Mutation

Tryptophan 220 replaced by Phenylalanine

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pTHSSe_60

Plasmid

#109254

Purpose

TALEsp2 stabilized promoter driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

TALEsp2 stabilized promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_59

Plasmid

#109253

Purpose

TALEsp1 stabilized promoter driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

TALEsp1 stabilized promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP

Plasmid

#67789

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

Insert

MMLV-LTR-GFP

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pOSV00170

Plasmid

#222934

Purpose

Constitutive GFP expression for Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

GFP(Sp)

Use

Synthetic Biology

Expression

Bacterial

Promoter

Phyperspank

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDest-659 (attR4-attR3)

Plasmid

#161883

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-676 (attR4-attR3)

Plasmid

#161888

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCC28

Plasmid

#128284

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, sopC

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBSDONR P4r-P2

Plasmid

#121527

Purpose

Gateway Entry Vector for Multisite Gateway Cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDJSVT87

Plasmid

#109302

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm)

Plasmid

#48886

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pVoPo-02

Plasmid

#187837

Purpose

Backbone for translational reporter system in Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHCL150

Plasmid

#134456

Purpose

CmR, Para-popZ-rbs-msfGFPC-H3H4. Replicative plasmid for the expression of bait. C-terminal GFP fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

rbs-msfGFPC-H3H4

Promoter

Para

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSAP

Plasmid

#206429

Purpose

A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Expression

Mammalian

Available Since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pKmK.I3R

Plasmid

#125065

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1R

Plasmid

#125063

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to LAC4

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1L

Plasmid

#125030

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to LAC4

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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