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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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DNMT3L-dCas9

Plasmid

#126587

Purpose

DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3L-dCas9-NLS

Expression

Mammalian

Available since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159872

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAN19-3xFLAG-ccvTIR1-NOSt

Plasmid

#108546

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Publication

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Mutation

Changed F79 to G

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_n-1] (GB1208)

Plasmid

#75409

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [D1_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

Plasmid

#75132

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pProEX-Af2Sir2

Plasmid

#8380

Depositor

Cynthia Wolberger

Publication

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Tags

His

Expression

Bacterial

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br

Plasmid

#13255

Depositor

Titia de Lange

Publication

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-44 + 96-399 and the point muta…

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Publication

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1-dTomato

Plasmid

#175278

Purpose

AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

Depositor

Wei Xu

Publication

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1; dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

dTomato (from bidirectional promoter)

Promoter

bidirectional TRE promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBOH-MHL.SUZ12

Plasmid

#125172

Purpose

Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

SUZ12 (SUZ12 Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

Plasmid

#132962

Purpose

Firefly-luciferase single-strand annealing assay cloning backbone

Depositor

Gang Bao

Publication

Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Expression

Mammalian

Mutation

Stop sequence between luciferase gene

Promoter

CMV

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Publication

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ7

Plasmid

#51443

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

Depositor

David Liu

Publication

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER-CXCR4

Plasmid

#42052

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

CXCR4 shRNA (CXCR4 )

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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