We narrowed to 39,215 results for: vit;

1. pDONR221_SLC39A14

   Plasmid

   #132105

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A14 (SLC39A14 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTRE Tight ArchT-GFP

   Plasmid

   #79627

   Purpose

   Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

   Depositor

   Yasunori Hayashi

   Article

   Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

   Insert

   ArchT
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ArchT is a more sensitive version of Arch (archae…

   Promoter

   Tet responsive Ptight promoter

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1P POLD1_1

   Plasmid

   #160791

   Purpose

   Suppress POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLD1_1
   

   Use

   Lentiviral

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1P REV3L_2

   Plasmid

   #160798

   Purpose

   Suppress REV3L

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shREV3L_2
   

   Use

   Lentiviral

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P REV3L_1

   Plasmid

   #160797

   Purpose

   Suppress REV3L

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shREV3L_1
   

   Use

   Lentiviral

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-TRE3G_NT-GSDMD_I105N_

   Plasmid

   #201174

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Mutation

   aa1-276 only, I105N

   Promoter

   TRE3G

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-DNPH1

   Plasmid

   #203224

   Purpose

   For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   DNPH1 (DNPH1 Human)
   

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-1 3xHA-MKK7a1-EE

   Plasmid

   #47578

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK7a1-EE (Map2k7 Mouse)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Residues 73-420 of RefSeq sequence. Ser271 to Gl…

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. PLK1 gRNA (BRDN0001145759)

   Plasmid

   #76457

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

11. ROCK1 gRNA (BRDN0001145904)

    Plasmid

    #77594

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTER E2F3shRNA human

    Plasmid

    #66885

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 3 (E2F3 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1 Tet

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

13. MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

    Plasmid

    #75136

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCardinal-Rab4a-7

    Plasmid

    #56174

    Purpose

    Localization: Ras GTPase, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Rab4a (RAB4A Human)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ROCK1 gRNA (BRDN0001146798)

    Plasmid

    #77595

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. non-targeting control gRNA (BRDN0001146709)

    Plasmid

    #80240

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

    Plasmid

    #75135

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLK1 gRNA (BRDN0001144995)

    Plasmid

    #76459

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRII-Blunt-U6-HES7-STOP-gRNA

    Plasmid

    #130933

    Purpose

    gRNA for tag human HES7

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7-STOP-gRNA (HES7 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO.1B POLE1_2

    Plasmid

    #160765

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO.1B POLE1_1

    Plasmid

    #160763

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PLK1 gRNA (BRDN0001144743)

    Plasmid

    #76458

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMSCV-GSDMD_NoCys-Myc

    Plasmid

    #201169

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Mutation

    C39A, C57A, C77A, C122A, C265A, C192A

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFPN1-N-gamma-tubulin (1-333)

    Plasmid

    #87858

    Purpose

    Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Fragment from aa 1 to 333

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLK1 gRNA (BRDN0001147678)

    Plasmid

    #76460

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126778

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FusXTBE-1397-C

    Plasmid

    #201398

    Purpose

    Expresses C terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FusXTBE-1397-N

    Plasmid

    #201397

    Purpose

    Expresses N terminus half of DddAtox in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSP64T acvr1ba*

    Plasmid

    #185409

    Purpose

    Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    acvr1ba (acvr1ba Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Expression

    Mammalian

    Mutation

    changed Threonine 206 to Aspartic acid

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. ADCK4 gRNA (BRDN0001145019)

    Plasmid

    #76560

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PTK6 gRNA (BRDN0001149364)

    Plasmid

    #76490

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK6

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK6 (PTK6 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PIP5K1A gRNA (BRDN0001147757)

    Plasmid

    #77963

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PIP5K1A gRNA (BRDN0001147420)

    Plasmid

    #77964

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PIP5K1B gRNA (BRDN0001147624)

    Plasmid

    #77083

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1B (PIP5K1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 496 pSG5L HA RB del651 (NAAIRS)

    Plasmid

    #10726

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del651 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 651 to 656 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

37. 500 pSG5L HA RB del685 (NAAIRS)

    Plasmid

    #10729

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del685 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 685 to 690 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

38. pT2EFCRET(#324)

    Plasmid

    #184059

    Purpose

    cyclofen-inducible CRE activation in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transient transgenesis

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Xl-EF1a

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p270 pCMV-CRE-M-AR(LNCaP)

    Plasmid

    #12496

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M AR (AR Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    Thr 868 Ala in AR-LBD

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

40. non-targeting control gRNA (BRDN0001145269)

    Plasmid

    #80249

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge