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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 4501 - 4520 of 6562 results

  4. pcDNA3.1-dCas9-CibN

    Plasmid
    #60552
    Purpose
    Expresses dCas9-CibN in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CibN
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60552-plasmid-map-sequence-id-97051

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  9. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60620-plasmid-map-sequence-id-99574

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  11. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Plasmid
    #68337
    Purpose
    Streptococcus thermophilus LMD-9 CRISPR 3 Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    St3Cas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68337-plasmid-map-sequence-id-125198

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  13. Lenti-BAT-RED

    Plasmid
    #20674
    Depositor
    Tannishtha Reya
    Insert
    Wnt reporter dsRED
    Tags
    NLS
    Expression
    Bacterial
    Available since
    May 26, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  17. PB-PE

    Plasmid
    #162796
    Purpose
    All-in-one prime editor piggyBac transposon
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162796-plasmid-map-sequence-id-320034

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  18. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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Showing: 4501 - 4520 of 6562 results