We narrowed to 8,727 results for: pcDNA3

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pcDNA3.2 mir-1-1 reporter cel-let-7

Plasmid

#46648

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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NC11 pCDNA3.1 VENUS WTX K292N

Plasmid

#36966

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX K292N (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

K292N

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
NC8 pCDNA3.1 VENUS WTX S286D

Plasmid

#36963

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286D (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286D

Promoter

CMV

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
Bcr del oligo pCDNA3.1/HisB

Plasmid

#36423

Depositor

John Groffen, Nora Heisterkamp

Article

Yi et al BMC Biochem. 2011 Feb 10;12:8.

Insert

BCR (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

Deletion of amino acid residues 2-63

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
NC7 pCDNA3.1 VENUS WTX S286A

Plasmid

#36962

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286A (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
NC10 pCDNA3.1 VENUS WTX T289A

Plasmid

#36965

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX T289A (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

T289A

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
NC9 pCDNA3.1 VENUS WTX S286E

Plasmid

#36964

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286E (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286E

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A,S77A)

Plasmid

#24262

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A,S90A) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine changed Serine 77 t…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74D,S77D)

Plasmid

#24263

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D,S90D) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid changed Serin…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
3501 pcDNA3 Bid p15 mIII.4

Plasmid

#8781

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p15 mIII.4 (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT 121-476

Plasmid

#112707

Purpose

Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 2-120

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-492)/pcDNA3.1

Plasmid

#111454

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-492), C643A

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R466A)-Venus

Plasmid

#138199

Purpose

FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub-RLuc8N-NES

Plasmid

#138213

Purpose

Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

Plasmid

#138299

Purpose

Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub(T/A)-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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