We narrowed to 5,055 results for: cre encoding plasmid

1. SuExp His-Myc-mPLD4

   Plasmid

   #201185

   Purpose

   Protein expression plasmid for recombinant His-Myc tag mouse PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (Pld4, Pld4, Pld4 Mouse)
   

   Use

   Tags

   His-Myc, Factor Xa cleavable

   Expression

   Mammalian

   Mutation

   cytosolic and transmembrane domain truncated; add…

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn1 YFP-CaRhAC

   Plasmid

   #101721

   Purpose

   Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Insert

   YFP CaRhAC
   

   Use

   AAV

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRiboI-Sec-APEX2m

   Plasmid

   #176845

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MFE23-ZZ

   Plasmid

   #223553

   Purpose

   MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

   Depositor

   Jean Gariepy

   Article

   Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

   Insert

   pET302-MFE23-ZZ
   

   Use

   Tags

   HA tag and HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAND1-T2A-dTom-PGK-Neo

   Plasmid

   #209838

   Purpose

   Tomato reporter plasmid targeting the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

   Insert

   HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid

    #175184

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. OKSIM

    Plasmid

    #24603

    Depositor

    Jose Cibelli

    Article

    Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

    Insert

    Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Oct4-2A-KLF4-2A-Sox2-IRES-Myc

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid

    #178330

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.GFAP.iGluSnFR3.v857.SGZ

    Plasmid

    #178337

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid

    #196218

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196227

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid

    #178331

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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