We narrowed to 73,935 results for: LAS

1. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156429

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   DELTA1-265

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

   Plasmid

   #197502

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

   Plasmid

   #92139

   Purpose

   Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-EGFP-Tau E14

   Plasmid

   #46907

   Purpose

   expresses EGFP tagged Tau E14 in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau E14 (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

   Plasmid

   #185917

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

   Plasmid

   #208289

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA; mutations in iSpyMac

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 ERG R367K

   Plasmid

   #118626

   Purpose

   Overexpresses ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG ( R367K DNA Binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   CMV

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. V5-ERG-R367K

   Plasmid

   #118624

   Purpose

   Expresses V5 tagged ERG R367K (DNA binding mutant) protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG (R367K DNA binding mutant) (ERG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R367K

   Promoter

   EF1a

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-EGFP-Tau E14 P301L

    Plasmid

    #46909

    Purpose

    expresses EGFP tagged Tau E14 P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau E14 P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L AND all 14 S/P or T/P amino acid residues (…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-EGFP-Tau AP P301L

    Plasmid

    #46906

    Purpose

    expresses EGFP tagged Tau AP P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau AP P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L AND all 14 S/P or T/P amino acid residues (…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid

    #197509

    Purpose

    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Expression

    Mutation

    ABE8e mutations in TadA and nSpCas9(D10A)

    Promoter

    Cbh

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169192

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Unspecified

    Tags

    6His-TEV

    Expression

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid

    #174516

    Purpose

    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

    Promoter

    GlnS and lpp

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge