We narrowed to 11,554 results for: Vars;

1. p35S::IRE1a

   Plasmid

   #135232

   Purpose

   constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   IRE1a (IRE1A Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY71-PT7-RiboJ-sfGFP-MGapt

   Plasmid

   #129119

   Purpose

   Expresses a fluorescent reporter for the quantification of cell-free protein expression.

   Depositor

   Matthew Lux

   Article

   Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

   Insert

   Superfolder GFP with Malachite Green aptamer
   

   Use

   Synthetic Biology

   Tags

   His6 and RiboJ Insulator

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnEA-vH_His-TEV-DSep1

   Plasmid

   #174493

   Purpose

   bacterial expression of Drosophila Sep1

   Depositor

   Manos Mavrakis

   Article

   Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

   Insert

   Sep1 (Sep1 Fly)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPKm-293

   Plasmid

   #105817

   Purpose

   pcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTAD

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   TET DBD and MTAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEMS2131

   Plasmid

   #111895

   Purpose

   ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPKm- 226

   Plasmid

   #90497

   Purpose

   pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PIF3-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_PSTPIP1-1/1

   Plasmid

   #91453

   Purpose

   Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PSTPIP1-1/1 (PSTPIP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mKO-Fibrillarin-7

   Plasmid

   #57842

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEMS1964

   Plasmid

   #82574

   Purpose

   High copy number plasmid containing Ple305 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple305
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P13L

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S235F

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_PIK3R1-1/1

    Plasmid

    #91274

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R1-1/1 (PIK3R1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

    Plasmid

    #159863

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_NEDD9-1/1

    Plasmid

    #91498

    Purpose

    Protein expression and purification of human SH3 domain construct NEDD9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEDD9-1/1 (NEDD9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUGW-GFP-2-cut reporter

    Plasmid

    #131669

    Purpose

    Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    GFP-2-cut reporter
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHL 1917

    Plasmid

    #53024

    Purpose

    AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    mCherry
    fimB (fimB E. coli)
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pCon and pLlacO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-h-mmPtch1-B-NNQ-HA-GFP

    Plasmid

    #121164

    Purpose

    Expresses mmPtch1-B NNQ variant

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, N…

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-Cre sgAdam10 v3

    Plasmid

    #158034

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159848

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge