We narrowed to 9,479 results for: ef1

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pJL306

Plasmid

#243703

Purpose

A piggyBac vector containing the NDUFB9 coding sequence (CDS) flanked by the 5' and 3' UTRs of the Actb gene

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

ACTB 5'UTR-NDUFB9 CDS-ACTB 3'UTR (NDUFB9 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHIV-H2B-eGFP

Plasmid

#91776

Purpose

Expresses H2B protein tagged with eGFP

Depositor

Maria Pia Cosma

Article

Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

Insert

Human Histone H2B / eGFP (H2BC11 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-SYP-HRP

Plasmid

#117186

Purpose

Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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iSpyMac

Plasmid

#155014

Purpose

Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

Insert

iSpyMac

Expression

Mammalian

Mutation

PID grafted onto SpyCas9 N-terminal domain, R221K…

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGFP-LacI

Plasmid

#179510

Purpose

Expresses EGFP-LacI in mammalian cells

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

LacI

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9i

Plasmid

#141249

Purpose

Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJL056

Plasmid

#177168

Purpose

Expresses wildtype MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-Rab12

Plasmid

#198471

Purpose

lentiviral expression of GFP-Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab12 (RAB12 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

Plasmid

#180587

Purpose

FdCd

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Cre

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMV_AA088

Plasmid

#216104

Purpose

Knockout, EF1a-driven Cas9 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRIF Flag

Plasmid

#41550

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

Trif (TICAM1 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pMCB306-GFP-Rab29

Plasmid

#198472

Purpose

lentiviral expression of GFP-Rab29

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab29 (RAB29 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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