Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 17,914 results for: REV

Showing: 11401 - 11450 of 17914 results

  1. pCAG-hPPP3R2

    Plasmid
    #179163
    Purpose
    Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179163-plasmid-map-sequence-id-352820

    Enlarge

  2. pCAG-mPpp3r2

    Plasmid
    #179161
    Purpose
    Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179161-plasmid-map-sequence-id-352819

    Enlarge

  11. pX330_ACTB-e1 sgRNA / hSpCas9

    Plasmid
    #172830
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172830-plasmid-map-sequence-id-341086

    Enlarge

  12. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid
    #172831
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172831-plasmid-map-sequence-id-341083

    Enlarge

  13. pX330_Camk2a sgRNA / hSpCas9

    Plasmid
    #172839
    Purpose
    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172839-plasmid-map-sequence-id-341073

    Enlarge

  14. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid
    #172829
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172829-plasmid-map-sequence-id-340926

    Enlarge

  15. pX330_ACTB-i3 sgRNA / hSpCas9

    Plasmid
    #172827
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172827-plasmid-map-sequence-id-340921

    Enlarge

  16. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172826-plasmid-map-sequence-id-340920

    Enlarge

  17. Ash1-5p

    Plasmid
    #169712
    Purpose
    Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-480…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169712-plasmid-map-sequence-id-334784

    Enlarge

  18. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

    Enlarge

  19. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

    Enlarge

  20. GFP-MIOX only

    Plasmid
    #166683
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    GFP-MIOX
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166683-plasmid-map-sequence-id-329938

    Enlarge

  21. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

    Enlarge

  22. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166673-plasmid-map-sequence-id-329825

    Enlarge

  28. eNS2B47NS3 S135A

    Plasmid
    #155318
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155318-plasmid-map-sequence-id-311249

    Enlarge

  29. pKM5

    Plasmid
    #154166
    Purpose
    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel2 (long isoform)
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154166-plasmid-map-sequence-id-309405

    Enlarge

  30. pKM28

    Plasmid
    #154165
    Purpose
    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel1A
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154165-plasmid-map-sequence-id-309403

    Enlarge

  31. pAA2

    Plasmid
    #154162
    Purpose
    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    16 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154162-plasmid-map-sequence-id-309382

    Enlarge

  32. pAA1

    Plasmid
    #154161
    Purpose
    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    12 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154161-plasmid-map-sequence-id-309344

    Enlarge

  33. pKM62

    Plasmid
    #154160
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 wt NF-κB sites were replaced with Rel2-specific…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154160-plasmid-map-sequence-id-309343

    Enlarge

  34. pKM59

    Plasmid
    #154158
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Enhancer for the Drosophila melanogaster Metchnik…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154158-plasmid-map-sequence-id-309332

    Enlarge

  35. pKM52

    Plasmid
    #154155
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154155-plasmid-map-sequence-id-309312

    Enlarge

  36. pKM51

    Plasmid
    #154154
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154154-plasmid-map-sequence-id-309306

    Enlarge

  42. p5E-161K enhancer-NR2F2-MUT

    Plasmid
    #138371
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    161k human enhancer with conserved NR2F2 binding sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    NR2F2 sites mutated
    Promoter
    none
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138371-plasmid-map-sequence-id-272006

    Enlarge

  43. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid
    #138372
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    human 161K human enhancer with ETS bind sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    ETS factor binding sites mutated
    Promoter
    NONE
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138372-plasmid-map-sequence-id-272008

    Enlarge

  44. pFA0057

    Plasmid
    #131784
    Purpose
    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer GFP cassette guide (gGFP) and 5' sgRNA
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131784-plasmid-map-sequence-id-258729

    Enlarge

  45. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

    Enlarge

Showing: 11401 - 11450 of 17914 results