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We narrowed to 14,582 results for: eng

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Showing: 4561 - 4580 of 14582 results

  1. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Publication
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  3. pCEP4-BG505.SOSIP-gp160

    Plasmid
    #123273
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123273-plasmid-map-sequence-id-235236

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  4. pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

    Plasmid
    #111504
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111504-plasmid-map-sequence-id-214283

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  5. p-mCherry-C1-Phobos

    Plasmid
    #98166
    Purpose
    blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct Phobos gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98166-plasmid-map-sequence-id-182715

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  9. AAVS1-eTLR

    Plasmid
    #214737
    Purpose
    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-eTLR (PPP1R12C Human, CMV, Anguilla japonica, influenza, Bovine, Rabbit, Synthetic, Chicken)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214737-plasmid-map-sequence-id-425005

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  10. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184249-plasmid-map-sequence-id-370367

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  11. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154105-plasmid-map-sequence-id-304581

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  13. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Publication
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  14. pcDNA3-Rs7327-RBD-8h

    Plasmid
    #161823
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161823-plasmid-map-sequence-id-316772

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  16. pNJB91

    Plasmid
    #51519
    Purpose
    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51519-plasmid-map-sequence-id-78693

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  17. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  19. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

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Showing: 4561 - 4580 of 14582 results