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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,291 results for: lat

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Showing: 4561 - 4580 of 36291 results

  3. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Publication
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201922-plasmid-map-sequence-id-395413

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  4. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid
    #192448
    Purpose
    ExRai-AMPKAR targeted to the mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Publication
    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mito-ExRai-AMPKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192448-plasmid-map-sequence-id-379142

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  7. AICSDP-50: NPM1-mEGFP

    Plasmid
    #109122
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    109122-plasmid-map-sequence-id-209780

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  9. pET-alpha-synuclein-BiFC

    Plasmid
    #87855
    Purpose
    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation
    Depositor
    Dan Mulvihill
    Publication
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    alpha-synuclein-VC155
    alpha-synuclein-VN154
    Tags
    C-terminal fragment of mVenus BiFC protein and N-…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87855-plasmid-map-sequence-id-175017

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  10. pCMV6-XL4 ASXL1 (1-1304)

    Plasmid
    #74244
    Purpose
    Cancer-associated ASXL1 truncation mutant in mammalian expression vector
    Depositor
    Anjana Rao
    Publication
    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ASXL1 (ASXL1 Human)
    Expression
    Mammalian
    Mutation
    Contains ASXL1 amino acids 1-1304
    Promoter
    CMV
    Available since
    Dec. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Amara, Susan Ingram
    Publication
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFPQS
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid
    #78101
    Purpose
    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    rTetR
    Expression
    Mammalian
    Promoter
    pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78101-plasmid-map-sequence-id-146271

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  18. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid
    #140978
    Purpose
    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaz-Rluc8 (GNAZ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140978-plasmid-map-sequence-id-300661

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  19. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  20. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Publication
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101849-plasmid-map-sequence-id-200249

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Showing: 4561 - 4580 of 36291 results