We narrowed to 6,403 results for: siae

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan

Plasmid

#232099

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Nat

Plasmid

#232100

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Hyg

Plasmid

#232095

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Expression

Yeast

Promoter

Z3

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-His3MX6

Plasmid

#41604

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-QFGal4-SV40pA

Plasmid

#155126

Purpose

In vitro transcription of QFGal4 mRNA

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Expression in zebrafish

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJO629

Plasmid

#104342

Purpose

Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

Insert

pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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LT2_14_GlyOx

Plasmid

#177711

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pKMV-VioD

Plasmid

#65350

Purpose

systhesis of vioD gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioD

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pKMV-VioB

Plasmid

#65348

Purpose

systhesis of vioB gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioB

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMM4

Plasmid

#170359

Purpose

Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PCYC1_SEC61_mCherry_LEU2_TCYC1

Expression

Yeast

Promoter

PCYC1

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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