We narrowed to 4,275 results for: Abo;

1. pLX304-BCL-XL-Y195F

   Plasmid

   #203573

   Purpose

   Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 195 to Phenylalanine for partial…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HS22 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135219

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HS22 (Homer1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HS38 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135221

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HS38 (Homer1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-SpCas9

   Plasmid

   #130968

   Purpose

   3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   3xHA-NLS-SpCas9
   

   Use

   CRISPR

   Tags

   3x HA, EGFP, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3294_pETcon-SARS2-RBD_Omicron-BA1

   Plasmid

   #184408

   Purpose

   yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304-BCL-XL-F97W

   Plasmid

   #203572

   Purpose

   Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 97 to Tryptophan for partia…

   Promoter

   CMV

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-His6-en1dFnCas9GFP

   Plasmid

   #201950

   Purpose

   Expression of en1dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en1dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1369R on FnCas9

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-en15dFnCas9GFP

   Plasmid

   #201951

   Purpose

   Expression of en15dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en15dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1603H on FnCas9

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304-BCL-XL-499R

    Plasmid

    #203574

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5- tagged

    Expression

    Mammalian

    Mutation

    silent point mutations introduced to make it resi…

    Promoter

    CMV

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458-3xHA-en31FnCas9

    Plasmid

    #201949

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en31FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    G1243T, E1369R, E1603H on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458-3xHA-en15FnCas9

    Plasmid

    #201948

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en15FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1603H on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid

    #201955

    Purpose

    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX304-BCL-XL-499/500R

    Plasmid

    #203575

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    silent point mutations to make resistant shRNA 49…

    Promoter

    CMV

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge