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Showing: 441 - 460 of 13210 results

  4. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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  14. pMH-Cas9-gate

    Plasmid
    #113742
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113742-plasmid-map-sequence-id-238953

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  17. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  18. pZeo-Cas9-gate

    Plasmid
    #113744
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113744-plasmid-map-sequence-id-219786

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  19. pSAG1::CAS9-U6::sg290860-6

    Plasmid
    #59855
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.
    Depositor
    David Sibley
    Article
    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229
    Insert
    CRISPR sg290860-6
    Use
    CRISPR; ; toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59855-plasmid-map-sequence-id-94714

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Showing: 441 - 460 of 13210 results