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Plasmid#179909PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-c-Met
Plasmid#31784DepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-mCherryCXCR4-W
Plasmid#200102PurposeLentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminusDepositorInsertCXCR4 (CXCR4 Human)
UseCRISPR and LentiviralTagsExpressionMutationsilent mutations (c.180C>T, 438G>A, 495C>…PromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-moxGFP
Plasmid#207744PurposeExpression of the Cre recombinase linked to a moxGFP fluorescent markerDepositorInsertCre-PGK-moxGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-oScarlet
Plasmid#137135PurposeViral expression of oScarletDepositorHas ServiceAAV8InsertoScarlet
UseAAVTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC
Plasmid#64874Purposelentiviral vector with Flag, HA tagsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH NICD1
Plasmid#86500PurposeMammalian expression of Myc tagged NICD1DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMycExpressionMammalianMutationCytoplasmic domain only (AA 1753-2531)PromoterEF1aAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NucGFP
Plasmid#184706PurposeLentiviral transfer plasmid for expression of NucGFPDepositorInsertNucGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1α-Dest
Plasmid#133299PurposeThe pT3-EF1α destination vector without any insert which can be used for LR reaction.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1amCherry-2A-OsTIR1-W
Plasmid#159296PurposeLentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) systemDepositorInsertOsTIR
UseLentiviralTagsExpressionMammalianMutationPromoterhuman EF1a promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbChT
Plasmid#45186DepositorHas ServiceAAV9InsertpAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
UseAAV; Adeno-associated viralTagsExpressionMammalianMutationPromoterhuman EF1aAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY
Plasmid#45185DepositorHas ServiceAAV9InsertpAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
UseAAV; Adeno-associated viralTagsExpressionMammalianMutationPromoterhuman EF1aAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
Plasmid#32627DepositorInsertDonor + Sce BFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-Tet-on 3G
Plasmid#118592PurposeExpresses Tet-on 3G from the EF1Alpha promoterDepositorInsertTet-on 3G
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
UseTagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->GssmCherry-PNE
Plasmid#162157PurposeLentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cellsDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only