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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,029 results for: CAG

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Showing: 4581 - 4600 of 10029 results

  4. pCAT077

    Plasmid
    #180511
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180511-plasmid-map-sequence-id-359731

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  5. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

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  6. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174554-plasmid-map-sequence-id-354966

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  7. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  8. pH6-TEV-spTorA-GFP(C)

    Plasmid
    #169041
    Purpose
    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.
    Depositor
    Siegfried Musser
    Publication
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    H6-spTorA-GFP
    Tags
    6xHis, TEV cleavage site, and spTorA (signal pept…
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169041-plasmid-map-sequence-id-346121

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  13. pmCellFree_KA1_SARS-COV-2_NSP4

    Plasmid
    #169379
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP4 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP4
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    Aug. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    169379-plasmid-map-sequence-id-339562

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  14. pmCellFree_KA1_SARS-COV-2_NSP12

    Plasmid
    #169385
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP12
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169385-plasmid-map-sequence-id-336500

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  15. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

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  16. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

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  17. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

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  18. pmCellFree_KA1_SARS-COV-2_NSP15

    Plasmid
    #169388
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP15
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169388-plasmid-map-sequence-id-336873

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  19. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169398-plasmid-map-sequence-id-336872

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  20. pmCellFree_KA1_SARS-COV-2_NSP8

    Plasmid
    #169382
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP8
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169382-plasmid-map-sequence-id-336870

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Showing: 4581 - 4600 of 10029 results