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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 4581 - 4600 of 16276 results

  1. pCAG_iPE-C

    Plasmid
    #214735
    Purpose
    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG_iPE-C_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214735-plasmid-map-sequence-id-425000

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  6. MERTK

    Plasmid
    #25178
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    axl2.571.864 (MERTK Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Publication
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  18. FXR1

    Plasmid
    #25341
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FXR1_25 (FXR1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  20. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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Showing: 4581 - 4600 of 16276 results