239,768 results

1. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Promoter

   dSV40 Promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHrD-IRES-Fluc

   Plasmid

   #194250

   Purpose

   Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

   Depositor

   Samson Jacob

   Article

   McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   human rDNA promoter and beginning of 5' ETS …

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX307 P2A GFP Luciferase

   Plasmid

   #193674

   Purpose

   Constitutive lentiviral expression of Luciferase

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ChromosomesMembranesandCytoskeleton

   Plasmid

   #62450

   Purpose

   MXS_chaining vector with 4 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::H2B-TagBFPx3-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::mCherryx3-linker-betaActin-bGHpA
   CMV::Citrinex3-linker-alphaTubulin-bGHpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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