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173,176 results

Showing: 6901 - 6930 of 173176 results

  4. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

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  5. PIP4K2AA

    Plasmid
    #42494
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIP4K2AA (PIP4K2A Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    42494-plasmid-map-sequence-id-398289

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  8. AAV-CamKIIa-jGCaMP8f-WPRE (AAV5)

    Viral Prep
    #176750-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only

  11. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  19. TFORF2594

    Plasmid
    #144048
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BHLHE41 (BHLHE41 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144048-plasmid-map-sequence-id-400508

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  20. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available Since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  24. pLNHA-C1-HsSmaug2_Q

    Plasmid
    #147285
    Purpose
    Mammalian Expression of HsSmaug2
    Depositor
    Elisa Izaurralde
    Insert
    HsSmaug2 (SAMD4B Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the seqeuence giv…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147285-plasmid-map-sequence-id-421013

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  25. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid
    #216165
    Purpose
    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mCherry with C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216165-plasmid-map-sequence-id-437747

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  26. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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Showing: 6901 - 6930 of 173176 results