We narrowed to 34,994 results for: CaS

1. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

   Plasmid

   #233039

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

   Plasmid

   #166873

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA array presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

   Plasmid

   #186987

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, Gluc crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

   Plasmid

   #140199

   Purpose

   Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

   Plasmid

   #101104

   Purpose

   Encodes dCas9(N) fused to VEGFR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

   Plasmid

   #114069

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid

    #140197

    Purpose

    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNase deactivated

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124431

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Zebrafish (if expression level is too high in zeb…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

    Plasmid

    #168216

    Purpose

    Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    SV40 NLS SpCas9
    

    Use

    Lentiviral

    Tags

    HA, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid

    #187954

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

    Plasmid

    #165445

    Purpose

    Expresses ACX, rPB(8kD) in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rPB(8kD)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid

    #192665

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid

    #110547

    Purpose

    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

    Depositor

    Ichiro Matsumura

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pBAD

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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