We narrowed to 10,236 results for: ada

1. pCMV_NOPlight1

   Plasmid

   #195578

   Purpose

   Expresses NOPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

   Insert

   NOPLight1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas12b(RuvC)

   Plasmid

   #186450

   Purpose

   Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12b
   

   Use

   CRISPR

   Mutation

   E848A, D977A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRFLIP38-AtLEA4-5

   Plasmid

   #178189

   Purpose

   SED1 biosensor for expression in Saccharomyces cerevisiae

   Depositor

   Cesar Cuevas-Velazquez, Jose Dinneny

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-KG-Itch/2793

   Plasmid

   #11434

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   Itch (Itch Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HUWE1-sgRNA-1

   Plasmid

   #86924

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA1 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

7. BirA-Myc_N_MYC

   Plasmid

   #167715

   Purpose

   Expresses MYC in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   MYC (MYC Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA SF-HERC2 F2

   Plasmid

   #55785

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

10. LentiCRISPR v2-sgAMPK alpha1 clone1

    Plasmid

    #162119

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5HT6-YC3.60

    Plasmid

    #48339

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
    

    Tags

    YC3.60

    Expression

    Mammalian

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Fireworks GFP[PTC-] (AVA2598)

    Plasmid

    #85445

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC_N_IRF8

    Plasmid

    #167763

    Purpose

    Expresses IRF8 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    IRF8 (IRF8 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FKBP-GFP-NAP1

    Plasmid

    #208862

    Purpose

    Stably express GFP-tagged NAP1 for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191156

    Purpose

    Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEX-CFP-hLC3WT

    Plasmid

    #24985

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fireworks RFP[PTC-] (AVA2515)

    Plasmid

    #85443

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid

    #213965

    Purpose

    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TS MS-Sensor

    Plasmid

    #199788

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TS MS-Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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