We narrowed to 14,321 results for: cas9

Showing: 4601 - 4620 of 14321 results

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SaCas9 GFP V3

Plasmid

#226962

Purpose

CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Nanog_2

Plasmid

#174871

Purpose

CRISPR vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Publication

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

CRISPR

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9-NG

Plasmid

#171692

Purpose

Expresses nSpCas9-NG in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9-NG

Use

CRISPR

Expression

Mammalian

Mutation

D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Publication

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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SpyCas9-AIDx

Plasmid

#135343

Purpose

Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Publication

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9-AsiA

Plasmid

#158063

Purpose

Expressing CasTA of dCas9 fused AsiA

Depositor

Harris Wang

Publication

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA

Expression

Bacterial

Promoter

TetO

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Publication

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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peSpCas9-J23109

Plasmid

#113152

Purpose

Plasmid constitutively expressing eSpCas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpCas9

Expression

Bacterial

Mutation

K810A & K1003A & R1060A

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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peSpdCas9-J23109

Plasmid

#113153

Purpose

Plasmid constitutively expressing eSpdCas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpdCas9

Expression

Bacterial

Mutation

D10A & K810A & H840A & K1003A & R…

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Jacquere (split-vector) (Lentiviral Prep)

Viral prep

#247026-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from Jacquere (split-vector) (#247026). In addition to the viral particles, you will also receive purified Jacquere (split-vector) plasmid DNA.

Depositor

John Doench, David Root

Publication

Drepanos et al Cell Genom. 2026 May 13;6(5):101190. doi

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-spCas9

Plasmid

#231404

Purpose

Expresses spCas9 from hSyn promoter

Depositor

Arjen Boender, Larry Young

Publication

Schimmer et al Nat Commun. 2026 Feb 20;17(1):1932. doi:

Insert

hSYN promoter (RIC8B Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSYN

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-evoCDA1

Plasmid

#209042

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

evoCDA1-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-nSpCas9-TadA8e

Plasmid

#209044

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TadA8e-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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nCas9-SpRY_P2A-mCherry

Plasmid

#219935

Purpose

Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-SpRY_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Publication

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Publication

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ANLN_sgRNA

Plasmid

#183874

Purpose

pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ANLN sgRNA spacer (ANLN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_Actb sgRNA / hSpCas9

Plasmid

#172832

Purpose

Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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