We narrowed to 5,861 results for: SPI

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SpyTag-RBD

Plasmid

#159999

Purpose

Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pg-EGFP-HIF-2alpha

Plasmid

#87207

Purpose

N-terminally EGFP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-2a (EPAS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.1

Plasmid

#173476

Purpose

Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF10_HA_BASU

Plasmid

#153980

Purpose

Express Orf10 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF3a_HA_BASU

Plasmid

#153994

Purpose

Express Orf3a gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Orf3a (ORF3a Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF6_HA_BASU

Plasmid

#153984

Purpose

Express Orf6 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf6 (ORF6 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-WT

Plasmid

#114037

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

Plasmid

#61841

Purpose

mammalian expression of cyclin B1 non-degradable L45A mutant

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

cyclin B1 (CCNB1 Human)

Tags

2xSTREP and 3xFLAG

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pTwist-WIV1-CoV

Plasmid

#164438

Purpose

Encodes WIV1-CoV Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF8_HA_BASU

Plasmid

#153982

Purpose

Express Orf8 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp14_HA_BASU

Plasmid

#153993

Purpose

Express NSP14 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE-YEE

Plasmid

#107687

Purpose

Expresses dLbCpf1-BE-YEE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YEE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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CIB-VP16 (B1014)

Plasmid

#92036

Purpose

Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 (intact NLS) with VP16 activ…

Expression

Mammalian

Promoter

T7

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp9_HA_BASU

Plasmid

#153986

Purpose

Express NSP9 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp5_HA_BASU

Plasmid

#153987

Purpose

Express NSP5 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp5 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_N_HA_BASU

Plasmid

#153989

Purpose

Express N gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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